Seizure Control by Low-Intensity Ultrasound in Mice with Temporal Lobe Epilepsy

BACKGROUND: Temporal lobe epilepsy (TLE) is the most common form of focal epilepsy. Recent studies have demonstrated that ultrasound stimulation can inhibit spontaneous recurrent seizures and improve behavioral outcomes for rodents with TLE. However, the exact underlying mechanism for inhibition of TLE via ultrasound stimulation remains unknown.

METHODS: In this study, low-intensity pulsed ultrasound stimulation (LIPUS) and low-intensity continuous ultrasound stimulation (LICUS) and concurrent local field potentials (LFPs) in the CA3 field of the hippocampus were recorded in the kainite-induced mouse model of TLE. The power spectrum and the phase-amplitude coupling in the LFPs were quantitatively analyzed.

RESULTS: This study found three significant changes in LFPs after ultrasound stimulation: (i) the intensity of the power spectrum in the low frequency (<10 Hz) was significantly decreased (p < 0.01); (ii) the phase amplitude coupling strength between slow (delta-, theta-, and alpha-frequency bands) and fast (gamma frequency bands) neural oscillations were weakened (p < 0.01); (iii) the interval between seizures was significantly increased (p < 0.01).

CONCLUSIONS: These results indicate that the seizures of temporal lobe epilepsy can be effectively inhibited by ultrasound stimulation, and this effect is independent of ultrasound type (pulsed or continuous).

Using Connectomics to Gain Insight into Epilepsy

Abnormalities in structural brain networks and how brain regions communicate may underlie a variety of disorders, including epilepsy, which is one focus of a two-part Special Issue on the Brain Connectome in Brain Connectivity, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers.

This second special issue includes the article entitled “Effective Connectivity within the Default Mode Network in Left Temporal Lobe Epilepsy: Findings from the Epilepsy Connectome Project,” –with results reported from the Epilepsy Connectome Project–contributed by Mary Meyerand, Jeffrey Binder and colleagues from University of Wisconsin-Madison, Medical College of Wisconsin (Milwaukee), and Froedtert Hospital (Milwaukee). Individuals with epilepsy can have memory impairment. Therefore, the researchers studied the default mode network that is a collection of brain regions involved in memory function. The results showed a difference in these memory-related connections between healthy individuals and individuals with epilepsy. The researchers identified an excitatory connection association with inhibition of formation of the left hippocampal region in patients with temporal lobe epilepsy.

The Special Issues were led by Guest Editors Brent Munsell, PhD, College of Charleston (SC), Guorong Wu, PhD, University of North Carolina at Chapel Hill, Leonardo Bonilha, MD, PhD, Medical University of South Carolina (Charleston), and Paul Laurienti, MD, PhD, Wake Forest School of Medicine (Winston-Salem, NC).

Researchers ID Factors Predictive of Naming Decline After Epilepsy Surgery

Paper- and online-based externally validated nomograms are effective in predicting naming decline after temporal lobe surgery in patients with epilepsy, according to study results published in Neurology. Factors predictive of postsurgical naming decline in this patient population included side of surgery, age at epilepsy onset, age at surgery, sex, and education, and preoperative naming score.

A total of 719 patients with epilepsy who underwent temporal lobe epilepsy surgery at the Cleveland Clinic were included in the study. In addition, the investigators enrolled an external validation cohort of 138 patients who also underwent temporal lobe surgery at Columbia University Medical Center, Emory University School of Medicine, or University of Washington School of Medicine.

Groundbreaking Stem Cell Brain Implant Helps Fight Epilepsy in Rats

Severe epilepsy is very difficult to treat, but an experimental approach involving implanted stem cells in the brain represents a groundbreaking way to potentially stop seizures for good.

Carried out by researchers at Texas A&M University, the technique has yet to be tried on human subjects, but has proven highly successful on rats. Rats given the implants suffered 70 percent fewer seizures than those without. That figure could drop further with additional research.

The investigation is the first study of its kind. It demonstrated that grafting certain cells derived from human-induced pluripotent stem cells into the brain can help alleviate seizures, as well as improve brain function. The work targets temporal lobe epilepsy (TLE), the most common type of epilepsy in which seizures originate from the brain’s hippocampus. TLE is a non-genetic form of epilepsy, often resulting from an incident such as a traumatic head injury, brain infections, or fever-related seizures during childhood.

Experiences of Self-Conscious Emotions in Temporal Lobe Epilepsy

Self-conscious emotions (SCEs) with a negative valence (such as shame and guilt) or a positive valence (such as pride) are moral emotions that emerge from self-reflection and self-evaluation processes in social contexts. In some neurologic and psychiatric disorders, experiences of SCEs are dysregulated.

Relative to controls, patients with TLE were more likely to experience negative-valence SCEs to a higher extent and positive SCEs to a lesser extent. The patients who experienced negative-valence SCEs to a higher extent (rather than to a lesser extent) had a higher frequency of seizures, more severe anxiety and depressive symptoms, and a greater prevalence of anxiety and depressive disorders. Furthermore, patients who experienced positive-valence SCEs to a lesser extent (rather than to a higher extent) displayed a higher level of anxiety. Lastly, differences in experiences of SCEs by patients with TLE were associated with a lower quality of life.

In conclusion, experiences of SCEs can be dysregulated in patients with TLE. This dysregulation is linked to the patients’ clinical and psychological symptoms and quality of life. In this context, SCEs might be a target of interest in the management of epilepsy.

Professor Catherine Christian and Jiang Li

Effects of Epilepsy on Neural Activity in Mice Fluctuate with Reproductive Cycle

Mice with epilepsy have altered patterns of neuron activity in the portion of the brain that controls the reproductive endocrine system, University of Illinois researchers report in a new study. Furthermore, the differences in neuron activity in female mice fluctuate across the reproductive cycle, the team found.

The study, which tracked hormone levels and activity in specific neurons that trigger the release of reproductive hormones in mice with epilepsy, demonstrates that the effects of epilepsy on other areas of the brain may not be as static as researchers have thought, said study leader Catherine Christian, a professor of molecular and integrative physiology at Illinois. The study was published in the journal eNeuro. Jiang Li, a graduate student at the University of Illinois, was the lead author of the study.

People with temporal lobe epilepsy – the most common type affecting adults – also have high rates of problems with their reproductive endocrine systems, though the connection is poorly understood.

Theory of Mind Impairment in Focal Versus Generalized Epilepsy

Theory of Mind (ToM) is a critical component of social cognition, and thus, its impairment may adversely affect social functioning and quality of life. Recent evidence has suggested that it is impaired in epilepsy. What is not clear, however, is whether it is related to particular types of epilepsy or other factors.

Researchers undertook this study to explore ToM in patients with focal versus those with generalized epilepsy, the particular pattern of ToM deficits, and the potential influence of antiepileptic medication load. The sample included 149 adults: 79 patients with epilepsy (34 with generalized epilepsy and 45 with focal epilepsy) and 70 healthy controls.

Theory of Mind tasks included:

  1. comprehension of hinting
  2. comprehension of sarcasm and metaphor
  3. comprehension of false beliefs and deception
  4. recognition of faux pas
  5. a visual ToM task in cartoon form

We found significant ToM impairment in the group with focal epilepsy relative to the performance of both the healthy group and the group with generalized epilepsy on all tasks, with the exception of faux pas, on which the group with generalized epilepsy also performed more poorly than the healthy group. Additionally, early age at seizure onset, but not antiepileptic drug (AED) load, was associated with ToM performance.

These findings suggest that focal temporal and frontal lobe, but not generalized, epilepsies were associated with impaired theory of mind. This may reflect the neuroanatomical abnormalities in the relevant neuronal networks and may have implications for differential cognitive-behavioral interventions based on epilepsy type.

Whole-Brain Connectome Maps Teach Artificial Intelligence to Predict Epilepsy Outcomes

Medical University of South Carolina (MUSC) neurologists have developed a new method based on artificial intelligence that may eventually help both patients and doctors weigh the pros and cons of using brain surgery to treat debilitating seizures caused by epilepsy. This study, which focused on mesial temporal lobe epilepsy (TLE), was published in the September 2018 issue of Epilepsia. Beyond the clinical implications of incorporating this analytical method into clinicians’ decision making processes, this work also highlights how artificial intelligence is driving change in the medical field.

Despite the increase in the number of epilepsy medications available, as many as one-third of patients are refractory, or non-responders, to the medication. Uncontrolled epilepsy has many dangers associated with seizures, including injury from falls, breathing problems, and even sudden death. Debilitating seizures from epilepsy also greatly reduce quality of life, as normal activities are impaired.

Epilepsy surgery is often recommended to patients who do not respond to medications. Many patients are hesitant to undergo brain surgery, in part, due to fear of operative risks and the fact that only about two-thirds of patients are seizure-free one year after surgery. To tackle this critical gap in the treatment of this epilepsy population, Dr. Leonardo Bonilha and his team in the Department of Neurology at MUSC looked to predict which patients are likely to have success in being seizure free after the surgery.

Neurology Department Chief Resident Dr. Gleichgerrcht explains that they tried “to incorporate advanced neuroimaging and computational techniques to anticipate surgical outcomes in treating seizures that occur with loss of consciousness in order to eventually enhance quality of life”. In order to do this, the team turned to a computational technique, called deep learning, due to the massive amount of data analysis required for this project.

Meta-analysis Shows General Pattern Of Cognitive Dysfunction In Frontal Lobe Epilepsy

Frontal lobe epilepsy (FLE) is associated with cognitive problems, especially in areas related to frontal lobe functioning as executive functions, attention, and motor skills, but with impact on memory and psychosocial adaptation. Deficits are similar in both adults and children with FLE, although no studies have compared adult and pediatric performance in the same study. The aim of this research was to analyze the existing evidence concerning the cognition in adults and children with FLE. A random effect meta-analysis was used using Cohen’s d, and the confidence interval for each cognitive factor was calculated. The results in the meta-analysis show a general pattern of cognitive dysfunction in FLE, especially in functions related to the frontal lobe, with an influence of the duration and the age at onset of epilepsy, as well as the age of the sample used. In addition, researches in this type of epilepsy are heterogeneous, with too many different sampling and methodological characteristics, which is not a standard format for reporting clinical sample characterization, making it difficult to study FLE in depth.

Unusual Ictal Propagation Patterns Suggesting Poor Prognosis After Temporal Lobe Epilepsy Surgery

OBJECTIVE: The objective of this study was to investigate unusual ictal propagation patterns in patients with drug-resistant temporal lobe epilepsy (TLE) and reveal their electrophysiological, neuroimaging, and prognostic properties after surgery.

METHODS: Among 248 patients with TLE who underwent scalp video-electroencephalographic (EEG) monitoring, 24 patients with ‘switch of lateralization’ or ‘bilateral asynchrony’ in at least one of their seizures (9.3%) were analyzed retrospectively. The postoperative outcome was determined in 16 patients who had undergone epilepsy surgery.

RESULTS: All but 5 of the included patients had hippocampal sclerosis (HS) as their magnetic resonance imaging (MRI) findings. Twelve out of 16 patients (75%) who had surgery were seizure-free for at least 1?year. Nine out of 12 patients (75%) with good outcome had unilateral interictal EEG discharges in temporal regions whereas 3 out of 4 patients with poor outcome had bilateral temporal interictal spiking (p?=?0.018).

CONCLUSION: Unusual ictal propagation patterns are not always related to poor prognosis after surgery in patients with temporal lobe epilepsy. Patients with unilateral interictal spiking in the temporal region tend to have good outcome despite these unusual patterns. These patterns can also be seen in patients with temporal lobe epilepsy with other etiologies besides the well-known HS in MRI.