Article published by Baylor College of Medicine
Glioma is one of the most aggressive malignant primary brain tumors. A common feature of glioma is the presence of localized, intermittent seizures referred to as glioma-related epilepsy, which is known to promote tumor growth. However, the mechanism involved at the molecular level is still not clear.
A team led by researchers at Baylor College of Medicine reports in the journal Neuron that the tumors from seizure patients can interfere with the ability of surrounding neurons to handle potassium, an important ion in neuronal communication. The disruption of this normal neural function drives seizures, which favor the progression of the disease.
“Our research involved both human patients and animal models. We started our investigation by analyzing data collected from human patients, then we modeled what we found in animal models and finally we went back to human patients. In collaboration with Dr. Ganesh Rao, Marc J. Shapiro Professor and Chair of Neurosurgery at Baylor, we applied single-cell RNA sequencing to determine tumor gene expression and analyzed intraoperative recordings of brain activity of human glioma, as well as other techniques,” said first author Rachel Naomi Curry, a graduate student in Dr. Benjamin Deneen’s lab at Baylor.
The team found that patients who have seizures have increased expression of genes involved in formation of neuronal connections or synapses. As they moved to study these genes in more detail in animal models, the researchers identified one of the genes, IGSF3, as the driver that mediated seizures in glioma.
“In collaboration with [former CURE Epilepsy grantee] Dr. Jeff Noebels, Cullen Trust for Health Care Endowed Chair in Neurogenetics and Director of Blue Bird Circle Developmental Neurogenetics Laboratory at Baylor, we dug deeper to understand the mechanism that leads to seizures. We discovered that IGSF3 disrupts the ability of tumor cells to handle potassium,” Curry said. “Normally, after neurons fire, astrocytes take up potassium in their surrounding environment. But IGSF3 suppresses the ability of these cells to uptake potassium, which leads to its accumulation and then to seizures.”
“When we looked at other genes that handle potassium, we found that there is a major loss of these genes in tumor cells of patients that have seizures. This is very specific to patients with seizures,” said Deneen, professor and Dr. Russell J. and Marian K. Blattner Chair of neurosurgery and director of the Center for Cancer Neuroscience at Baylor. He also is the corresponding author of the work.